Inside of Primary Containment (in the Biosafety Cabinet)

• Stop work
• Remove soiled PPE
• Exit the BSC
• Wait 3 minutes for aerosols to settle
• Don fresh PPE
• Cover the spill with paper towels
• Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
• Collect materials as biohazardous waste
• Remove PPE and wash hands before exiting the lab
• Notify supervisor and EH&S Biological Safety Office (352-392-1591)

Outside of Containment (in the Lab)

• Stop work and alert co-workers
• Remove PPE and exit the lab
• Post a sign to prohibit lab entry
• Wait 30 minutes for aerosols to settle
• Don fresh PPE
• Cover the spill with paper towels
• Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
• Collect materials as biohazardous waste
• Remove PPE and wash hands before exiting the lab
• Notify supervisor and EH&S Biological Safety Office (352-392-1591)

Inside a Centrifuge

• Close centrifuge lid
• Remove PPE and exit the lab
• Post a sign to prohibit entry
• Wait 30 minutes for aerosols to settle
• Centrifuge basin:
  • Don fresh PPE
  • Cover the spill with paper towels
  • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
  • Collect materials as biohazardous waste
  • Remove PPE and wash hands before exiting the lab
  • Notify supervisor and EH&S Biological Safety Office (352-392-1591)
• Rotors/buckets/cups:
  • Don fresh PPE
  • Prepare 1:10 bleach bath in the BSC
  • Transfer contaminated item to the BSC
  • Submerge in bleach bath (wait a contact time of 30 minutes)
  • Discard byproducts as biohazardous waste.
  • Remove PPE and wash hands
  • Notify supervisor and EH&S Biological Safety Office (352-392-1591)