• Research Safety & Services
  • Occupational Safety & Risk Management
  • Facility Support Services
  • Inside of Primary Containment (in the Biosafety Cabinet)

    • Stop work
    • Remove soiled PPE
    • Exit the BSC
    • Wait 3 minutes for aerosols to settle
    • Don fresh PPE
    • Cover the spill with paper towels
    • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
    • Collect materials as biohazardous waste
    • Remove PPE and wash hands before exiting the lab
    • Notify supervisor and EH&S Biological Safety Office (352-392-1591)

    Outside of Containment (in the Lab)

    • Stop work and alert co-workers
    • Remove PPE and exit the lab
    • Post a sign to prohibit lab entry
    • Wait 30 minutes for aerosols to settle
    • Don fresh PPE
    • Cover the spill with paper towels
    • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
    • Collect materials as biohazardous waste
    • Remove PPE and wash hands before exiting the lab
    • Notify supervisor and EH&S Biological Safety Office (352-392-1591)

    Inside a Centrifuge

    • Close centrifuge lid
    • Remove PPE and exit the lab
    • Post a sign to prohibit entry
    • Wait 30 minutes for aerosols to settle
    • Centrifuge basin:
      • Don fresh PPE
      • Cover the spill with paper towels
      • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
      • Collect materials as biohazardous waste
      • Remove PPE and wash hands before exiting the lab
      • Notify supervisor and EH&S Biological Safety Office (352-392-1591)
    • Rotors/buckets/cups:
      • Don fresh PPE
      • Prepare 1:10 bleach bath in the BSC
      • Transfer contaminated item to the BSC
      • Submerge in bleach bath (wait a contact time of 30 minutes)
      • Discard byproducts as biohazardous waste.
      • Remove PPE and wash hands
      • Notify supervisor and EH&S Biological Safety Office (352-392-1591)

    Handling Biological Spills Handout