Biological Spill

Inside of Primary Containment (in the Biosafety Cabinet)

  • Stop work
  • Remove soiled PPE
  • Exit the BSC
  • Wait 3 minutes for aerosols to settle
  • Don fresh PPE
  • Cover the spill with paper towels
  • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
  • Collect materials as biohazardous waste
  • Remove PPE and wash hands before exiting the lab
  • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/

Outside of Containment (in the Lab)

  • Stop work and alert co-workers
  • Remove PPE and exit the lab
  • Post a sign to prohibit lab entry
  • Wait 30 minutes for aerosols to settle
  • Don fresh PPE
  • Cover the spill with paper towels
  • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
  • Collect materials as biohazardous waste
  • Remove PPE and wash hands before exiting the lab
  • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/

Inside a Centrifuge

  • Close centrifuge lid
  • Remove PPE and exit the lab
  • Post a sign to prohibit entry
  • Wait 30 minutes for aerosols to settle
  • Centrifuge basin:
    • Don fresh PPE
    • Cover the spill with paper towels
    • Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
    • Collect materials as biohazardous waste
    • Remove PPE and wash hands before exiting the lab
    • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/
  • Rotors/buckets/cups:
    • Don fresh PPE
    • Prepare 1:10 bleach bath in the BSC
    • Transfer contaminated item to the BSC
    • Submerge in bleach bath (wait a contact time of 30 minutes)
    • Discard byproducts as biohazardous waste.
    • Remove PPE and wash hands
    • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/
Handling Biological Spills Handout Biological Spill Kit Components