- Homepage
- Departments
- Research Safety & Services
- Biological Safety
- Biological Spills
Biological Spills
- Animal Research Safety
- Biological Safety
- Acute Biological Toxins
- Autoclaves
- Biohazard Project Registration
- Biohazardous Waste Disposal
- Biological Spills
- BioPath Program
- BloodBorne Pathogen Program
- NIH Guidelines Flowchart
- Plant Research & Greenhouses
- Recombinant & Synthetic Nucleic Acids
- Research Involving Flying Insects
- SARS-CoV-2/COVID-19 Specimen Research
- Select Agents
- Shipping and Transport of Biological Materials
- Vaccination for Research Personnel
- Chemical and Lab Safety
- Acids That Deserve Special Attention
- Chemical Exposures
- Chemical Hygiene Plan
- Chemical Inventory
- Chemical Spills
- Chemical Storage and Management
- Compressed Gas
- Controlled Substances & Pharmaceutical Products
- Cryogens
- Equipment Decontamination
- Equipment Purchase Approval
- First Aid Kit Info
- Hydrofluoric Acid
- Lab Safety Manual
- Lab Ventilation & Fume Hoods
- Lessons Learned
- Minors and Visitors in the Lab
- New Labs, Moving Labs & Closeouts
- Peroxide Forming Compounds
- Safety Surveys
- Signage & Stickers
- Standard Operating Procedures
- Signage
- Chemical Safety Information
- Gator TRACS
- Hazardous Waste Management
- Radiation and Laser Safety
- Report Lab Closeout
- Boating & Dive Safety
- Drones/UAS
- Ergonomics
- Industrial Hygiene & Occupational Safety
- Occupational Medicine
- Risk Management Insurance & Liability
- Building Codes Enforcement
- Building Emergency Coordinators
- ADA Assessment
- Certificate of Occupancy or Completion
- Applicable Building Codes for the State of Florida and the University of Florida
- Code Permit Fee Schedule
- Inspections
- Permit Application Process
- Permit Instructions
- Plan Submittal Guidelines
- Temporary Structures on Campus – Including Tents
- Chartfield Form Codes Permits
- Fire Plan Review and Inspection
- Pest Management
Inside of Primary Containment (in the Biosafety Cabinet)
- Stop work
- Remove soiled PPE
- Exit the BSC
- Wait 3 minutes for aerosols to settle
- Don fresh PPE
- Cover the spill with paper towels
- Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
- Collect materials as biohazardous waste
- Remove PPE and wash hands before exiting the lab
- Notify supervisor and EH&S Biological Safety Office (352-392-1591)
Outside of Containment (in the Lab)
- Stop work and alert co-workers
- Remove PPE and exit the lab
- Post a sign to prohibit lab entry
- Wait 30 minutes for aerosols to settle
- Don fresh PPE
- Cover the spill with paper towels
- Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
- Collect materials as biohazardous waste
- Remove PPE and wash hands before exiting the lab
- Notify supervisor and EH&S Biological Safety Office (352-392-1591)
Inside a Centrifuge
- Close centrifuge lid
- Remove PPE and exit the lab
- Post a sign to prohibit entry
- Wait 30 minutes for aerosols to settle
- Centrifuge basin:
- Don fresh PPE
- Cover the spill with paper towels
- Pour 1:10 (> 5000 ppm) bleach solution on the spill (start from the oter bounds and move inward)
- Collect materials as biohazardous waste
- Remove PPE and wash hands before exiting the lab
- Notify supervisor and EH&S Biological Safety Office (352-392-1591)
- Rotors/buckets/cups:
- Don fresh PPE
- Prepare 1:10 bleach bath in the BSC
- Transfer contaminated item to the BSC
- Submerge in bleach bath (wait a contact time of 30 minutes)
- Discard byproducts as biohazardous waste.
- Remove PPE and wash hands
- Notify supervisor and EH&S Biological Safety Office (352-392-1591)
Handling Biological Spills Handout | Biological Spill Kit Components |