Inside of Primary Containment (in the Biosafety Cabinet)

• Stop work
• Remove soiled PPE
• Exit the BSC
• Wait 3 minutes for aerosols to settle
• Don fresh PPE and gather the Biological Spill Kit
• Cover the spill with paper towels
• Pour 5000 ppm of NaClO (bleach) solution on the spill (start from the outer bounds and move inward). 
• Wipe surface clean. 
• Spray 10% ethanol solution to prevent corrosion and wipe surface clean
• Collect materials as biohazardous waste
• Remove PPE and wash hands before exiting the lab
• Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/

Outside of Containment (in the Lab)

• Stop work and alert co-workers
• Remove PPE and exit the lab
• Post a sign to prohibit lab entry
• Wait 30 minutes for aerosols to settle
• Don fresh PPE and gather the Biological Spill Kit
• Cover the spill with paper towels
• Pour 5000 ppm  of NaClO (bleach) solution on the spill (start from the outer bounds and move inward). Wipe surface clean. Use additional paper towels if needed
• Collect materials as biohazardous waste
• Remove PPE and wash hands before exiting the lab
• Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/

Inside a Centrifuge

• Close centrifuge lid
• Remove PPE and exit the lab
• Post a sign to prohibit entry
• Wait 30 minutes for aerosols to settle
• Centrifuge basin:
  • Don fresh PPE and gather the Biological Spill Kit
  • Cover the spill with paper towels
  • Pour 5000 ppm NaClO (bleach) solution on the spill (start from the outer bounds and move inward). Wipe surface clean.
  • Collect materials as biohazardous waste
  • Remove PPE and wash hands before exiting the lab
  • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident
• Rotors/buckets/cups:
  • Don fresh PPE and gather the Biological Spill Kit
  • Prepare 5000 ppm NaClO (bleach) bath in the biosafety cabinet (BSC)
  • Transfer contaminated item to the BSC
  • Submerge in bleach bath for 30 minutes
  • Discard byproducts as biohazardous waste.
  • Remove PPE and wash hands
  • Notify Supervisor and report at: ehs.ufl.edu/report-to-ehs/report-incident/