Autoclaves
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- Autoclaves
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Laboratory personnel must be aware of the potential for burns while handling hot sterilized items and use caution when removing them from autoclaves/sterilizers.
DOs
- Have a cart available to transport items to/from autoclave.
- Wear a lab coat and always use heat-resistant gloves to load/unload an autoclave.Gauntlets provide wrist and forearm protection for handling hot items.
- Ensure that the autoclave/sterilizer door is closed and locked prior to starting a cycle.
- Do not remove items from an autoclave until they have cooled.
- Use forceps or other tools to remove sharp instruments or broken glass from pans and autoclaves.
DON’Ts
- Do not leave biohazardous waste unattended. If the sterilizer is in use, please return the biohazardous waste to your laboratory.
- DO NOT change autoclave temperature to >250ºF.
- DO NOT shut off the autoclave at the end of your cycle.
- DO NOT adjust water/steam settings or valves.
- DO NOT autoclave liquids containing bleach, formalin, or glutaraldehyde.
- Do NOT autoclave flammable or volatile liquids.
- Do NOT autoclave radioactive materials.
- Do NOT autoclave items containing corrosives (acids, bases, or phenol).
- Do NOT OPEN the chamber door if water leaks from front of the autoclave. clogged lines, equipment malfunction or a failure in the steam supply may cause the autoclave chamber to fill with scalding water Failure to heed this warning may result in second- or third-degree burns from scalding water!