Lab Safety Survey Guidelines

Criteria for the Laboratory Safety Survey can be found in the

Acrobat Laboratory Safety Manual

Acrobat Biological Safety Manual

Acrobat Radiation Control Guide

Chemical Waste Management Guide


General Safety

  • Notice Board with Emergency Call list and hazard warning labels are posted at lab entrance(s) (provided by EH&S-Lab Safety 392-1591)
  • UF Laboratory Safety Manual is readily accessible
  • Chemical Spill Kit is available in each lab housing liquid chemicals
  • A First-Aid Kit is readily accessible; Calcium gluconate is available for labs working with hydrofluoric acid.
  • Safety Shower and plumbed Eyewash Unit are readily accessible to lab personnel
  • Airfoils and slots on chemical fume hoods are unobstructed; side panels are in place
  • Storage of chemicals and equipment in chemical fume hoods is kept to a minimum
  • Vacuum pumps are equipped with a belt and pulley guard, if applicable
  • Lab equipment is safely operated and maintained according to manufacturers directions

Personal Protective Equipment (PPE), Housekeeping, and Hygiene

  • PPE e.g., gloves, safety glasses/goggles, lab coats, thermal protection, etc. is available (stored clean and in good repair) and worn for the activities being conducted
  • Hearing protection is worn for high noise areas (e.g. sonicators, grinders)
  • Full coverage shoes with good sole grips are worn in the lab; no open-toed shoes (sandals) are allowed
  • Lab personnel are aware that contact lenses should not be worn in the labs
  • Respirator wearers are trained, fit tested, and registered with EH&S
  • Walkways and safety equipment are clear of obstructions
  • Work surfaces and benches are free of clutter to reduce risk of spills and accidents
  • Mechanical pipetting devices are used; pipetting by mouth is not allowed
  • Food and beverages are not permitted in the lab working area, or stored in lab refrigerators/freezers
  • Refrigerators, freezers, and microwaves are clearly labeled, e.g., “non-flammable storage only;” “no food or beverages” (provided by EH&S Lab Safety 392-1591)
  • PPE is removed before leaving the lab

Chemical Safety

  • Proper storage procedures are followed for all chemicals including labeling, capping/sealing, and proper container type/condition
  • Liquid chemicals are stored below shoulder height
  • Proper dating/storage/use/disposal procedures are followed for perchloric acid
  • Proper dating/storage/use/disposal procedures are followed for peroxide forming compounds

Lab waste disposal

  • Lab personnel are trained in EH&S Hazardous Materials Management and Contact EH&S for training dates
  • Outdated and unused chemicals are disposed of through EH&S Hazardous Materials Management
  • All hazardous waste containers are properly sealed and labeled with yellow EH&S labels
  • A Hazardous Waste Satellite Accumulation Area is designated and the requirements are posted (last page of the Chemical Waste Management Guide)
  • Biological Waste is decontaminated and disposed of properly
  • Broken glass is separated from regular waste stream and properly labeled as noted in University of Florida Policy for Disposal of Clean Lab Ware
  • Needles and small sharps are disposed of in red plastic ‘sharps’ box

Compressed gases

  • Compressed gas cylinders are adequately secured (even when empty) and stored by compatibility
  • Gas cylinders are securely transported using a hand truck
  • The UF Compressed Gas Rules are posted in a prominent location
  • Cylinders are stored away from heat sources
  • The regulator connection is leak tested after installation and before each use
  • Cylinders with no regulators must be capped (even when empty)
  • Contents of cylinders must be clearly labeled
  • Hydrostatic tests are current (cylinders have not been stored more than 5-10 years

Electrical Safety

  • Access to circuit breaker panel is unobstructed
  • Openings on breaker panel, receptacle boxes, etc. are sealed
  • Ground Fault Circuit Interrupters (GFCI) are used near sinks and wet areas
  • Electrical cords are checked periodically for fraying or damage
  • Lab appliances are plugged directly into electrical outlets (UL rated power surge protectors may be used when there is a lack of electrical outlets but extension cords cannot be used as a permanent power supply)

Fire Safety

  • A Fire Extinguisher is located near or in the lab
  • Large metal drums of flammable liquids are grounded during transfer
  • A maximum of 10 gallons of flammable liquids are stored in a lab; the excess is stored in a safety cabinet or safety cans up to a maximum total of 50 gallons
  • Flammable liquids over 1 gallon are stored in safety cans
  • Sprinkler heads are not blocked
  • Vents on flammable storage cabinets are sealed

Drug Enforcement Agency controlled substances: (DEA)

  • DEA permit is current (A DEA permit is required for controlled substances)
  • DEA use log and employee questionnaires are current for controlled substances on hand
  • Controlled substances are stored in secure location
  • Outdated or unwanted DEA substances are disposed of appropriately

Biological Materials

  • Is the following research registered with EH&S Biological Safety Office?
  • Recombinant DNA experimentation
  • Bio-Safety Level (BSL) of 2 or 3
  • State or federally regulated materials
  • Acute toxins (LD50 of ≤ 100 ug/kg body weight)
  • Unknown human and animal pathogens
  • Cell lines or cultures that have been immortalized with a virus or are primary human tumor cells
  • Human blood or other tissues that are known to be HIV positive (or positive for any human disease-causing virus or other agent), when used in research
  • Blood or other tissues from old world monkeys

Radiation Safety

Please see EH&S Radiation Control

Record keeping