LAB SAFETY
LIST
Criteria for the Laboratory Safety Survey can be found in the:
Chemical Waste Management Guide
General Safety
q Notice Board with Emergency Call list and hazard warning labels are posted at lab entrance(s) (provided by EH&S-Lab Safety 392-1591)
q UF Laboratory Safety Manual is readily accessible
q A Chemical Spill Kit is available in each lab housing liquid chemicals
q A First-Aid Kit is readily accessible; Calcium gluconate is available for labs working with hydrofluoric acid.
q Safety Shower and plumbed Eyewash Unit are readily accessible to lab personnel
q Airfoils and slots on chemical fume hoods are unobstructed; side panels are in place
q Storage of chemicals and equipment in chemical fume hoods is kept to a minimum
q Vacuum pumps are equipped with a belt and pulley guard, if applicable
q Lab equipment is
safely operated and maintained according to manufacturers directions
Personal Protective Equipment (PPE), Housekeeping, and Hygiene:
q PPE e.g., gloves, safety glasses/goggles, lab coats, thermal protection, etc. is available (stored clean and in good repair) and worn for the activities being conducted
q Hearing protection is worn for high noise areas (e.g. sonicators, grinders)
q Full coverage shoes with good sole grips are worn in the lab; no open-toed shoes (sandals) are allowed
q Lab personnel are aware that contact lenses should not be worn in the labs
q Respirator wearers are trained, fit tested, and registered with EH&S
q Walkways and safety equipment are clear of obstructions
q Work surfaces and benches are free of clutter to reduce risk of spills and accidents
q Spills are cleaned up promptly
q Mechanical pipetting devices are used; pipetting by mouth is not allowed
q Food and beverages are not permitted in the lab working area, or stored in lab refrigerators/freezers
q Refrigerators, freezers, and microwaves are clearly labeled, e.g., "non-flammable storage only;" "no food or beverages" (provided by EH&S Lab Safety 392-1591)
q PPE is removed before leaving the lab
Chemical
Safety:
q Proper storage procedures are followed for all chemicals including labeling, capping/sealing, and proper container type/condition
q Liquid chemicals are stored below shoulder height
q Proper dating/storage/use/disposal procedures are followed for perchloric acid
q Proper dating/storage/use/disposal procedures are followed for peroxide forming compounds
Lab
waste disposal:
q Lab personnel are trained in EH&S Hazardous Materials Management and training dates are available on EH&S Training Calendar
q Outdated and unused chemicals are disposed of through EH&S Hazardous Materials Management
q All hazardous waste containers are properly sealed and labeled with yellow EH&S labels
q A Hazardous Waste Satellite Accumulation Area is designated and the requirements are posted (last page of the Chemical Waste Management Guide)
q Biological Waste is decontaminated and disposed of properly
q Broken glass is separated from regular waste stream and properly labeled as noted in University of Florida Policy for Disposal of Clean Lab Ware
q Needles and small sharps are disposed of in red plastic ‘sharps’ box
Compressed
gases:
q Compressed gas cylinders are adequately secured (even when empty) and stored by compatibility
q Gas cylinders are securely transported using a hand truck
q The UF Compressed Gas Rules are posted in a prominent location
q Cylinders are stored away from heat sources
q The regulator connection is leak tested after installation and before each use
q Cylinders with no regulators must be capped (even when empty)
q Contents of cylinders must be clearly labeled
q Hydrostatic tests are current (cylinders have not been stored more than 5-10 years
Electrical Safety:
q Access to circuit breaker panel is unobstructed
q Openings on breaker panel, receptacle boxes, etc. are sealed
q Ground Fault Circuit Interrupters (GFCI) are used near sinks and wet areas
q Electrical cords are checked periodically for fraying or damage
q Lab appliances are plugged directly into electrical outlets (UL rated power surge protectors may be used when there is a lack of electrical outlets but extension cords cannot be used as a permanent power supply)
Fire Safety:
q A Fire Extinguisher is located near or in the lab
q Large metal drums of flammable liquids are grounded during transfer
q A maximum of 10 gallons of flammable liquids are stored in a lab; the excess is stored in a safety cabinet or safety cans up to a maximum total of 50 gallons
q Flammable liquids over 1 gallon are stored in safety cans
q Sprinkler heads are not blocked
q Vents on flammable storage cabinets are sealed
Drug Enforcement Agency (DEA) controlled
substances:
q DEA permit is current (A DEA permit is required for controlled substances)
q DEA use log and employee questionnaires are current for controlled substances on hand
q Controlled substances are stored in secure location
q Outdated or unwanted DEA substances are disposed of appropriately (contact EH&S Hazardous Materials Management for more information)
Biological Materials:
q Is the following research registered with EH&S Biological Safety Office?
q Recombinant DNA experimentation
q Bio-Safety Level (BSL) of 2 or 3
q State or federally regulated materials
q Acute toxins (LD50 of ≤ 100 ug/kg body weight)
q Unknown human and animal pathogens
q Cell lines or cultures that have been immortalized with a virus or are primary human tumor cells
q Human blood or other tissues that are known to be HIV positive (or positive for any human disease-causing virus or other agent), when used in research
q Blood or other tissues from old world monkeys
Radiation
Safety:
Please see EH&S Radiation
Control
Record
keeping:
q Material Safety Data Sheets (MSDS)
q A recent (within the last year) chemical inventory must be kept on file (email to labsafety@ehs.ufl.edu on annual basis)
q DEA records
q Bloodborne Pathogen Program/Exposure Control Plan
q Appropriate safety manuals e.g., UF Laboratory Safety Manual, UF Biological Safety Manual, UF Chemical Waste Management Guide, etc.